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Many families have been unable to find the bodies of their loved ones as the Kroonstad morgue closed over the weekend.
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The Railway Safety Regulator has instituted a board of inquiry to establish the root cause of the accident.
Three children were among the injured and recovering in hospital, the Free State department of health said.
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Authorities say that all survivors have been removed from the scene. Heavy duty machinery is on its way to enable the search for more bodies.
At least four people have died and around 100 others have been injured.